abstract：:Micromass cultures represent a convenient means of studying chondrocyte physiology in the context of a tridimensional culture model. In this study, we present the first ultrastructural analysis of the distribution and organization of the extracellular components in micromasses in comparison with their cartilaginous co...

journal_title：Microscopy research and technique

authors： Battistelli M,Borzì RM,Olivotto E,Vitellozzi R,Burattini S,Facchini A,Falcieri E

更新日期：2005-08-15 00:00:00

abstract：:The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual ...

journal_title：Microscopy research and technique

authors： Cruthers N,Carr D,Niven B,Girvan E,Laing R

更新日期：2005-08-01 00:00:00

abstract：:We summarize the molecular phenotype, diagnostic criteria, and the newest advances related to seeking the pathogenic mechanism(s) of sporadic inclusion-body myositis (s-IBM), a muscle disease usually of persons over age 50. On the basis of our research, several processes seem to be important in relation to the still-s...

journal_title：Microscopy research and technique

authors： Askanas V,Engel WK

更新日期：2005-07-01 00:00:00

abstract：:Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi...

journal_title：Microscopy research and technique

authors： Pauly JL,Allison EM,Hurley EL,Nwogu CE,Wallace PK,Paszkiewicz GM

更新日期：2005-06-01 00:00:00

abstract：:The intravacuolar network inside the parasitophorous vacuole of Toxoplasma gondii consists of an intricate system of membrane-limited tubules of uncertain role in parasite development. We propose that it is an important structural support to the maintenance of the parasites in the characteristic rosette arrangement of...

journal_title：Microscopy research and technique

authors： Magno RC,Lemgruber L,Vommaro RC,De Souza W,Attias M

更新日期：2005-05-01 00:00:00

abstract：:The mechanism of the candidacidal activity of histatins 3 and 5 (Hst) is still a matter of debate. Previous studies have indicated that Hst induce cell permeabilization, generation of reactive oxygen species (ROS) by mitochondria, inhibition of the respiratory chain, and energy-dependent cytotoxic release of ATP. On t...

journal_title：Microscopy research and technique

authors： Diaz G,Polonelli L,Conti S,Messana I,Cabras T,Putzolu M,Falchi AM,Fadda ME,Cosentino S,Isola R

更新日期：2005-04-01 00:00:00

abstract：:In a previous report we described the survival and contractile function of mouse embryonic stem cell-derived cardiomyocytes in the host retroperitoneum. To further understand the nature of embryonic stem cell-derived cardiomyocytes, the study assessed the synthesis of natriuretic peptides in ectopic myocardial tissues...

journal_title：Microscopy research and technique

authors： Johkura K,Cui L,Yue F,Nitta K,Takei S,Okouchi Y,Asanuma K,Ogiwara N,Sasaki K

更新日期：2005-03-01 00:00:00

abstract：:STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...

journal_title：Microscopy research and technique

authors： Murphy S,Ceballos SF,Mariotto G,Berdunov N,Jordan K,Shvets IV,Mukovskii YM

更新日期：2005-02-01 00:00:00

abstract：:Acrylic resin mixtures are commonly used to study microscopic sections of biological specimens, giving the advantage of good morphological preservation. Existing embedding protocols, however, are suitable for tissue blocks, not exceeding 1 mm in thickness. We have developed a protocol to embed larger specimens (up to ...

journal_title：Microscopy research and technique

authors： De Jonge HW,De Bakker MA,Verbeek FJ,Weijs WA

更新日期：2005-01-01 00:00:00

abstract：:Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-...

journal_title：Microscopy research and technique

authors： Byers MR,Maeda T,Brown AM,Westenbroek RE

更新日期：2004-12-01 00:00:00

abstract：:We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times th...

journal_title：Microscopy research and technique

authors： Barcellona ML,Gammon S,Hazlett T,Digman MA,Gratton E

更新日期：2004-11-01 00:00:00

abstract：:Broiler chicken and laying hen livers were fixed using a simple technique of in situ puncture perfusion of cacodylate-buffered fixative, which allowed characterisation of the fine structure of hepatic parenchyma, hepatocytes, bile ductules, and, in particular, the sinusoidal cells including endothelial, Kupffer, and I...

journal_title：Microscopy research and technique

authors： Ghoddusi M,Kelly WR

更新日期：2004-09-01 00:00:00

abstract：:We have examined cellular events at the early stages of periosteal chondrogenesis and osteogenesis induced by bone fracture, using a well-standardized rib fracture model of the mouse. The initial cellular event was recognized as considerable proliferation in the deeper layer referred to as the "cambium layer" of the p...

journal_title：Microscopy research and technique

authors： Li M,Amizuka N,Oda K,Tokunaga K,Ito T,Takeuchi K,Takagi R,Maeda T

更新日期：2004-07-01 00:00:00

abstract：:In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DR...

journal_title：Microscopy research and technique

authors： Oztürk G,Erdoğan E

更新日期：2004-06-15 00:00:00

abstract：:We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an impro...

journal_title：Microscopy research and technique

authors： Ibáñez-López C,Escobar I,Saavedra G,Martínez-Corral M

更新日期：2004-06-01 00:00:00

abstract：:Förster resonance energy transfer (FRET) allows one to study interactions between two fluorescently labeled molecules (donors and acceptors) at distances on the order of 5 nm. Many studies have described methods of how to measure the efficiency of FRET. However, few have addressed the question of how fluorescence from...

journal_title：Microscopy research and technique

authors： Neher RA,Neher E

更新日期：2004-06-01 00:00:00

abstract：:Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...

journal_title：Microscopy research and technique

authors： Abbro L,Lanubile R,Dini L

更新日期：2004-05-01 00:00:00

abstract：:The molecular basis of hearing is less well understood than many other senses. However, recent studies in Drosophila have provided some important steps towards a molecular understanding of hearing. In this report, we summarize these findings and their implications on the relationship between hearing and touch. In Dros...

journal_title：Microscopy research and technique

authors： Todi SV,Sharma Y,Eberl DF

更新日期：2004-04-15 00:00:00

abstract：:Epidemiologic studies have associated cardiovascular morbidity and mortality with ambient particulate air pollution. Particles smaller than 100 nm in diameter (ultrafine particles) are present in the urban atmosphere in very high numbers yet at very low mass concentration. Organs beyond the lungs are considered as tar...

journal_title：Microscopy research and technique

authors： Kapp N,Kreyling W,Schulz H,Im Hof V,Gehr P,Semmler M,Geiser M

更新日期：2004-04-01 00:00:00

abstract：:The field of quantitative analysis and subsequent mapping of the cerebral cortex has developed rapidly. New powerful tools have been applied to investigate large regions of complex folded gyrencephalic cortices in order to detect structural transition regions that might partition different cortical fields of disjunct ...

journal_title：Microscopy research and technique

authors： Schmitt O,Pakura M,Aach T,Hömke L,Böhme M,Bock S,Preusse S

更新日期：2004-03-01 00:00:00

abstract：:An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...

journal_title：Microscopy research and technique

authors： Mitome M,Bando Y,Golberg D,Kurashima K,Okura Y,Kaneyama T,Naruse M,Honda Y

更新日期：2004-02-15 00:00:00

abstract：:The nasal fluid is an easily accessible form of airway surface liquid. The objective of this study was to find a technically easy and reproducible method for sampling and analysis of this fluid. In a pilot study, several methods to carry out X-ray microanalysis of sub-microliter droplets were compared. Acceptable resu...

journal_title：Microscopy research and technique

authors： Vanthanouvong V,Roomans GM

更新日期：2004-02-01 00:00:00

abstract：:We report on a novel laser source, emitting high energy (20 nanoJoule) femtosecond pulses, in a broad spectrum (250 nm). This source is easily tuned from 950 to 1200 nm, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10-nm spectral width. ...

journal_title：Microscopy research and technique

authors： Deguil N,Mottay E,Salin F,Legros P,Choquet D

更新日期：2004-01-01 00:00:00

abstract：:We have summarized current knowledge on the aging of the immune system in three vertebrate groups: fish, amphibians and birds. Available data are few due to difficulties in studying ageing in natural populations and in accurately determining age. In all vertebrates, the most obvious evidence of the senescence of lymph...

journal_title：Microscopy research and technique

authors： Torroba M,Zapata AG

更新日期：2003-12-15 00:00:00

abstract：:The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group r...

journal_title：Microscopy research and technique

authors： Baccari GC,Raucci F,Di Fiore MM,Monteforte R

更新日期：2003-12-01 00:00:00

abstract：:A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units i...

journal_title：Microscopy research and technique

authors： Croce G,Frache A,Milanesio M,Viterbo D,Bavestrello G,Benatti U,Giovine M,Amenitsch H

更新日期：2003-11-01 00:00:00

abstract：:The aim of this report was to investigate the ultrastructure and three-dimensional organization of the pleural lymphatic stomata in the adult rabbit costal pleura by electron microscopy. A computer image processing system attached to the scanning electron microscopy was used to get statistical evaluation of the dimens...

journal_title：Microscopy research and technique

authors： Li YY,Li JC

更新日期：2003-10-15 00:00:00

abstract：:Golgi impregnations reveal a variety of dendritic morphologies amongst Kenyon cells in the mushroom bodies of Drosophila melanogaster. Different morphological types of Kenyon cells contribute axon-like processes to five divisions of the medial and vertical lobes. Four of these divisions have characteristic affinities ...

journal_title：Microscopy research and technique

authors： Strausfeld NJ,Sinakevitch I,Vilinsky I

更新日期：2003-10-01 00:00:00

abstract：:Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image mon...

journal_title：Microscopy research and technique

authors： Jacobs MD,Donaldson PJ,Cannell MB,Soeller C

更新日期：2003-09-01 00:00:00

abstract：:This report describes osteoclastic bone resorption around intraosseous fixation screws in rat and pig mandibles. These screws supported distraction devices and provided for neutral fixation following the distraction period. Progressive clinical instability of bicortical screws and radiographic and histologic evidence ...

journal_title：Microscopy research and technique

authors： Schulten AJ,Zimmermann CE,Glowacki J

更新日期：2003-08-15 00:00:00

abstract：:The morphological development of the vomeronasal organ (VNO) and accessory olfactory bulb (AOB) of the sheep from anlage to birth were studied by classical and histochemical methods using embryos and fetuses obtained from an abattoir with ages estimated from crown-to-rump length. Both VNO and AOB developed in a biolog...

journal_title：Microscopy research and technique

authors： Salazar I,Lombardero M,Alemañ N,Sánchez Quinteiro P

更新日期：2003-08-01 00:00:00

abstract：:The perivitelline space of mammalian oocytes changes in size and composition during preimplantation development. Often overlooked in the past, this space contains a hyaluronan-rich extracellular matrix prior to fertilization and a cortical granule envelope following release of the cortical granules at fertilization. T...

journal_title：Microscopy research and technique

authors： Talbot P,Dandekar P

更新日期：2003-07-01 00:00:00

abstract：:Pituitary-derived factors, including ACTH, have been widely implicated in initiating adrenal regeneration. However, recent work has demonstrated that adrenal regeneration is also modulated by adrenal nerves that extensively reinnervate the regenerating adrenal. Moreover, transection of the splanchnic nerve removes sen...

journal_title：Microscopy research and technique

authors： Ulrich-Lai YM,Fraticelli AI,Engeland WC

更新日期：2003-06-15 00:00:00

abstract：:Peroxisomes are essential organelles that may be involved in various functions, dependent on organism, cell type, developmental stage of the cell, and the environment. Until recently, peroxisomes were viewed as a class of static organelles that developed by growth and fission from pre-existing organelles. Recent obser...

journal_title：Microscopy research and technique

authors： Veenhuis M,Kiel JA,Van Der Klei IJ

更新日期：2003-06-01 00:00:00

abstract：:In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic...

journal_title：Microscopy research and technique

authors： Cooper TG,Yeung CH

更新日期：2003-05-01 00:00:00

abstract：:This article reviews the distribution of S100 proteins in the human peripheral nervous system. The expression of S100 by peripheral glial cells seems to be a distinctive fact of these cells, independently of their localization and their ability to myelinate or not. S100 proteins expressing cells include satellite cell...

journal_title：Microscopy research and technique

authors： Gonzalez-Martinez T,Perez-Piñera P,Díaz-Esnal B,Vega JA

更新日期：2003-04-15 00:00:00

abstract：:Term and preterm cervical ripening and dilatation have similarities with an inflammatory reaction. Since cell adhesion molecules are involved in this process, investigations on the expression of intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, vascular cell adhesion molecule-1, and platele...

journal_title：Microscopy research and technique

authors： Winkler M,Kemp B,Fischer DC,Ruck P,Rath W

更新日期：2003-03-01 00:00:00

abstract：:Neuropeptides are peptides with profound effects on the nervous system. The function of neuropeptides can be studied in detail in the stomatogastric nervous system (STNS). Neuropeptides are ubiquitously distributed in the STNS and it contains well-studied neural circuits that are strongly modulated by neuropeptides. T...

journal_title：Microscopy research and technique

authors： Skiebe P

更新日期：2003-02-15 00:00:00

abstract：:Historically, there has been much debate on the nature of infantile hemangiomas as either congenital malformations or benign neoplasms. Some vascular lesions that are present at birth and evidence no proliferative growth are considered to be congenital malformations; other post-natal vascular tumors pursue aggressive ...

journal_title：Microscopy research and technique

authors： Bell CD

更新日期：2003-02-01 00:00:00

abstract：:Central to the control of vascular resistance in the systemic and pulmonary circulation and at the solid organ level is the function of the endothelial-derived vasomediators, endothelin-1 (ET-1) and nitric oxide. Regulation of steady-state levels of ET-1 and endothelial nitric oxide synthase (eNOS) mRNAs represents an...

journal_title：Microscopy research and technique

authors： Mawji IA,Marsden PA

更新日期：2003-01-01 00:00:00